Review

polyclonal igg custom antibody (Boster Bio)

Name: polyclonal igg custom antibody
Brand: Boster Bio
Rating: 93 (4 reviews)

Boster Bio is a verified supplier

Boster Bio manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Boster Bio polyclonal igg custom antibody
Polyclonal Igg Custom Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal igg custom antibody/product/Boster Bio
Average 93 stars, based on 4 article reviews

polyclonal igg custom antibody - by Bioz Stars, 2026-02

93/100 stars

Images

Image Search Results

Myh9&10 TAL-cKO mice develop progressive kidney disease and show sexual dimorphism in morbidity. (A) Timeline depicting experimental protocol for Myh9&10 TAL-cKO (TAL-cKO) characterization. (B) Myh9&10 TAL-cKO (TAL-cKO) female mice fail to gain weight compared to littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ). At 12 weeks of age TAL-cKO females are significantly smaller than littermate controls and meet euthanasia criteria by 14 weeks of age. TAL-cKO male mice also fail to gain weight and are smaller than littermate controls starting at 12 weeks of age and remain significantly smaller at 16 and 20 weeks of age. Male TAL-cKO mice spontaneously die around 24-25 weeks of age. (C) Blood urea nitrogen (BUN) levels become progressively higher during disease progression in female and male TAL-cKO mice compared to littermate controls. Female and male TAL-cKO mice exhibit higher BUN starting at 9 weeks of age. The dotted line indicates normal BUN value (30mg/dL). (D) Serum creatinine levels in both female and male TAL-cKO mice become progressively higher compared to littermate controls. (E) Quantification of the tubular injury marker NGAL in the urine shows elevated concentration in both male (triangles) and female (circles) TAL-cKO mice as early as 9 weeks of age compared to littermate controls, and NGAL was further elevated at later time points in both female and male mice (n=6 each sex). (F) Quantification of CD3+ cell counts show immune cell infiltration at 13 weeks of age in female (circles) and male (triangles) and 20-week-old male TAL-cKO mice compared to littermate controls. (G) 24-hour urine samples collected from female and male TAL-cKO mice indicate higher urine acidity compared to littermate controls. Body weight was analyzed independently for each sex by age using an unpaired t test with Welch’s correction or Mann-Whitney test and graphed together. BUN and creatinine were analyzed independently for each sex by two-way ANOVA. Male and female urine NGAL data was combined and analyzed by two-way ANOVA with post-hoc Tukey’s test. Male and female CD3 data was combined and analyzed by two-way ANOVA with post-hoc Tukey’s test. Sample numbers for CD3+ counts can be found in materials and methods. Mean + SEM values are graphed with individual samples; significant p values are also listed on graphs. Sample numbers for serum and urine data at each timepoint can be found in the supplemental tables. Urine pH was analyzed independently for each sex and age using an unpaired t test with Welch’s correction or Mann-Whitney test.

Journal: Function

Article Title: Thick Ascending Limb Specific Inactivation of Myh9 and Myh10 Myosin Motors Results in Progressive Kidney Disease and Drives Sex-specific Cellular Adaptation in the Distal Nephron and Collecting Duct

doi: 10.1093/function/zqae048

Figure Lengend Snippet: Myh9&10 TAL-cKO mice develop progressive kidney disease and show sexual dimorphism in morbidity. (A) Timeline depicting experimental protocol for Myh9&10 TAL-cKO (TAL-cKO) characterization. (B) Myh9&10 TAL-cKO (TAL-cKO) female mice fail to gain weight compared to littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ). At 12 weeks of age TAL-cKO females are significantly smaller than littermate controls and meet euthanasia criteria by 14 weeks of age. TAL-cKO male mice also fail to gain weight and are smaller than littermate controls starting at 12 weeks of age and remain significantly smaller at 16 and 20 weeks of age. Male TAL-cKO mice spontaneously die around 24-25 weeks of age. (C) Blood urea nitrogen (BUN) levels become progressively higher during disease progression in female and male TAL-cKO mice compared to littermate controls. Female and male TAL-cKO mice exhibit higher BUN starting at 9 weeks of age. The dotted line indicates normal BUN value (30mg/dL). (D) Serum creatinine levels in both female and male TAL-cKO mice become progressively higher compared to littermate controls. (E) Quantification of the tubular injury marker NGAL in the urine shows elevated concentration in both male (triangles) and female (circles) TAL-cKO mice as early as 9 weeks of age compared to littermate controls, and NGAL was further elevated at later time points in both female and male mice (n=6 each sex). (F) Quantification of CD3+ cell counts show immune cell infiltration at 13 weeks of age in female (circles) and male (triangles) and 20-week-old male TAL-cKO mice compared to littermate controls. (G) 24-hour urine samples collected from female and male TAL-cKO mice indicate higher urine acidity compared to littermate controls. Body weight was analyzed independently for each sex by age using an unpaired t test with Welch’s correction or Mann-Whitney test and graphed together. BUN and creatinine were analyzed independently for each sex by two-way ANOVA. Male and female urine NGAL data was combined and analyzed by two-way ANOVA with post-hoc Tukey’s test. Male and female CD3 data was combined and analyzed by two-way ANOVA with post-hoc Tukey’s test. Sample numbers for CD3+ counts can be found in materials and methods. Mean + SEM values are graphed with individual samples; significant p values are also listed on graphs. Sample numbers for serum and urine data at each timepoint can be found in the supplemental tables. Urine pH was analyzed independently for each sex and age using an unpaired t test with Welch’s correction or Mann-Whitney test.

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Marker, Concentration Assay, MANN-WHITNEY

Hematoxylin and eosin (H&E) staining of female Myh9&10 TAL-cKO kidneys shows progressive histological changes. (A-C) Representative images from 9-week-old littermate control ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ) kidneys show healthy renal tissue in the cortical, corticomedullary, and medullary regions. (D-F) 9-week-old Myh9&10 TAL-cKO (TAL-cKO) mice have minimal tubular dilation and cellular density. (G-I) Representative images of 12-week-old controls for the cortical, corticomedullary, and medullary regions also show healthy renal tissue. (J-L) Evaluation of 12-week-old TAL-cKO female kidneys shows focal areas of tubular dilation and extensive damage with infiltrating cells in the corticomedullary and medullary regions Scale bars = 100µm. (n= 3-4 kidneys each).

Journal: Function

doi: 10.1093/function/zqae048

Figure Lengend Snippet: Hematoxylin and eosin (H&E) staining of female Myh9&10 TAL-cKO kidneys shows progressive histological changes. (A-C) Representative images from 9-week-old littermate control ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ) kidneys show healthy renal tissue in the cortical, corticomedullary, and medullary regions. (D-F) 9-week-old Myh9&10 TAL-cKO (TAL-cKO) mice have minimal tubular dilation and cellular density. (G-I) Representative images of 12-week-old controls for the cortical, corticomedullary, and medullary regions also show healthy renal tissue. (J-L) Evaluation of 12-week-old TAL-cKO female kidneys shows focal areas of tubular dilation and extensive damage with infiltrating cells in the corticomedullary and medullary regions Scale bars = 100µm. (n= 3-4 kidneys each).

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Staining, Control

Uromodulin (UMOD) expression and localization in Myh9&10 TAL-cKO mice are influenced by both Umod+/CreERT2 transgene and loss of MYH9&10 proteins. (A-D) Representative immunoblots from whole kidney lysates from female and male kidneys show changes in UMOD expression at 6 and 13 weeks. UMOD protein expression is lower in both male and female kidneys at 6 (A, B) and 13 (C, D) weeks of age compared to control kidneys (n=6 each sex). Tubulin (TUB) is loading control and L = Ladder Lane. (E) Immunoblots of whole kidney lysates from female control (UMOD+/+ and UMODcre/+) and TAL-cKO (TAL-cKO) mice at 6 weeks of age (upper panel) and 12 weeks of age (lower panel) showing changes in UMOD protein levels. (F) Quantification of immunoblots confirms lower UMOD protein levels at 6 and 12 weeks of age in UMODcre/+ and TAL-cKO kidneys compared to UMOD+/+ kidneys. (G) Quantitative PCR analysis of Umod gene expression in female (circles) and male (triangles) kidneys. Significantly lower gene expression is observed in Umodcre/+ kidneys compared to Umod+/+ kidneys at all ages analyzed, indicating that presence of the cre transgene reduces transcript levels. At 6 and 12 weeks of age, expression is also lower in TAL-cKO kidneys compared to Umod+/+ kidneys. At 9 weeks of age there is significantly higher gene expression in TAL-cKO kidneys compared to Umodcre/+ kidneys. In TAL-cKO kidneys, Umod gene expression is significantly higher between 6 and 9 weeks of age and significantly lower from 9 and 12 weeks of age (n≥5 kidneys per genotype and age). (H-I) Representative immunoblot shows that in 9-week-old TAL-cKO male and female kidneys UMOD protein expression is restored to control kidney levels (H, I). (J-M) Fluorescence immunostaining shows variable expression of UMOD in TAL tubules of female and male Myh9&10 TAL-cKO (TAL-cKO) kidneys at 9 weeks of age compared to littermate controls (Myh9f/f; Myh10f/f; Umod+/+). Tubules also show accumulation of UMOD while others show loss of UMOD protein. (N-Q) Representative images from immunostained 13-week old male and female kidneys show dilated tubules with increased UMOD accumulation as well tubules with loss of UMOD protein in TAL-cKO kidneys compared to controls. Scale bars = 50µm (n= 3 kidneys each). Immunoblot results (B, D, I) were normalized to controls (n=3 per blot, n=6 total) and analyzed by unpaired t test with either Welch’s correction or Mann-Whitney test. Immunoblots results (F) were normalized to controls (UMOD+/+) and analyzed independently by age using one-way ANOVA with post-hoc Dunnett’s T3 multiple comparisons test (n=3-6 kidneys each). Transcript data (G) was analyzed by two-way ANOVA with post hoc Tukey’s multiple comparisons test.

Journal: Function

doi: 10.1093/function/zqae048

Figure Lengend Snippet: Uromodulin (UMOD) expression and localization in Myh9&10 TAL-cKO mice are influenced by both Umod+/CreERT2 transgene and loss of MYH9&10 proteins. (A-D) Representative immunoblots from whole kidney lysates from female and male kidneys show changes in UMOD expression at 6 and 13 weeks. UMOD protein expression is lower in both male and female kidneys at 6 (A, B) and 13 (C, D) weeks of age compared to control kidneys (n=6 each sex). Tubulin (TUB) is loading control and L = Ladder Lane. (E) Immunoblots of whole kidney lysates from female control (UMOD+/+ and UMODcre/+) and TAL-cKO (TAL-cKO) mice at 6 weeks of age (upper panel) and 12 weeks of age (lower panel) showing changes in UMOD protein levels. (F) Quantification of immunoblots confirms lower UMOD protein levels at 6 and 12 weeks of age in UMODcre/+ and TAL-cKO kidneys compared to UMOD+/+ kidneys. (G) Quantitative PCR analysis of Umod gene expression in female (circles) and male (triangles) kidneys. Significantly lower gene expression is observed in Umodcre/+ kidneys compared to Umod+/+ kidneys at all ages analyzed, indicating that presence of the cre transgene reduces transcript levels. At 6 and 12 weeks of age, expression is also lower in TAL-cKO kidneys compared to Umod+/+ kidneys. At 9 weeks of age there is significantly higher gene expression in TAL-cKO kidneys compared to Umodcre/+ kidneys. In TAL-cKO kidneys, Umod gene expression is significantly higher between 6 and 9 weeks of age and significantly lower from 9 and 12 weeks of age (n≥5 kidneys per genotype and age). (H-I) Representative immunoblot shows that in 9-week-old TAL-cKO male and female kidneys UMOD protein expression is restored to control kidney levels (H, I). (J-M) Fluorescence immunostaining shows variable expression of UMOD in TAL tubules of female and male Myh9&10 TAL-cKO (TAL-cKO) kidneys at 9 weeks of age compared to littermate controls (Myh9f/f; Myh10f/f; Umod+/+). Tubules also show accumulation of UMOD while others show loss of UMOD protein. (N-Q) Representative images from immunostained 13-week old male and female kidneys show dilated tubules with increased UMOD accumulation as well tubules with loss of UMOD protein in TAL-cKO kidneys compared to controls. Scale bars = 50µm (n= 3 kidneys each). Immunoblot results (B, D, I) were normalized to controls (n=3 per blot, n=6 total) and analyzed by unpaired t test with either Welch’s correction or Mann-Whitney test. Immunoblots results (F) were normalized to controls (UMOD+/+) and analyzed independently by age using one-way ANOVA with post-hoc Dunnett’s T3 multiple comparisons test (n=3-6 kidneys each). Transcript data (G) was analyzed by two-way ANOVA with post hoc Tukey’s multiple comparisons test.

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Expressing, Western Blot, Control, Real-time Polymerase Chain Reaction, Fluorescence, Immunostaining, MANN-WHITNEY

Changes in ER chaperone expression and ER stress/unfolded protein response proteins are observed in Myh9&10 TAL-cKO mice. (A) Immunostained kidney sections from 13-week-old male and female Myh9&10 TAL-cKO (TAL-cKO) and littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ) show higher levels of the ER chaperone calreticulin (CALR) and accumulation within dilated tubules that also have uromodulin (UMOD) accumulation. CALR co-localizes with the accumulated UMOD in TAL-cKO kidneys. There are also dilated tubules with loss of UMOD that show varying levels of CALR expression. Scale bars = 20µm. (n=3 each). (B, C) Western blot analysis of whole kidney lysates shows higher CALR expression in 13-week-old male and female TAL-cKO mice compared to the littermate control kidneys (n=3 each). (D, E) Immunoblots of whole kidney lysates from 13-week-old male and female control and TAL-cKO kidneys show no changes in total calnexin (CANX) protein levels (~78kDa) (n=3 each). (F, G) Immunoblot analysis of whole kidney lysates from 13-week-old male and female control and TAL-cKO kidneys shows ER unfolded protein response (UPR) protein ATF6 levels (~105kDa) are significantly higher in female TAL-cKO kidneys compared to littermate control kidneys (n=6 each). (H, I) Immunoblot analysis of ER UPR response protein XBP1 in whole kidney lysates from 13-week-old TAL-cKO mice shows significantly lower XBP1 protein levels (~40kDa) (indicating apoptotic phase) compared to littermate control kidneys (n=6 each). Transferrin receptor (TFRC), tubulin (TUB), and Gapdh are loading controls and L = Ladder Lane. (J-Q) Super resolution images of 13-week-old control and TAL-cKO kidneys immunostained for UMOD and the endoplasmic reticulum (ER) tubule-associated protein reticulon 4 (RTN4). (J-M) In control kidney TAL cells, UMOD is enriched along the apical (luminal) membrane while RTN4 is present as intracellular puncta. (N-Q) In TAL-cKO kidneys, UMOD accumulates within TAL segments, the cells of which exhibit more intense RTN4 staining that appears to partially colocalize with intracellular UMOD, suggesting retention of UMOD within the ER. Scale bars: L, P = 20µm, M, Q (insets) = 5µm. Immunoblot results were normalized to controls in individual blots (n=3 per blot, n=3-6 total) and analyzed by unpaired t test with either Welch’s correction or Mann-Whitney test.

Journal: Function

doi: 10.1093/function/zqae048

Figure Lengend Snippet: Changes in ER chaperone expression and ER stress/unfolded protein response proteins are observed in Myh9&10 TAL-cKO mice. (A) Immunostained kidney sections from 13-week-old male and female Myh9&10 TAL-cKO (TAL-cKO) and littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ) show higher levels of the ER chaperone calreticulin (CALR) and accumulation within dilated tubules that also have uromodulin (UMOD) accumulation. CALR co-localizes with the accumulated UMOD in TAL-cKO kidneys. There are also dilated tubules with loss of UMOD that show varying levels of CALR expression. Scale bars = 20µm. (n=3 each). (B, C) Western blot analysis of whole kidney lysates shows higher CALR expression in 13-week-old male and female TAL-cKO mice compared to the littermate control kidneys (n=3 each). (D, E) Immunoblots of whole kidney lysates from 13-week-old male and female control and TAL-cKO kidneys show no changes in total calnexin (CANX) protein levels (~78kDa) (n=3 each). (F, G) Immunoblot analysis of whole kidney lysates from 13-week-old male and female control and TAL-cKO kidneys shows ER unfolded protein response (UPR) protein ATF6 levels (~105kDa) are significantly higher in female TAL-cKO kidneys compared to littermate control kidneys (n=6 each). (H, I) Immunoblot analysis of ER UPR response protein XBP1 in whole kidney lysates from 13-week-old TAL-cKO mice shows significantly lower XBP1 protein levels (~40kDa) (indicating apoptotic phase) compared to littermate control kidneys (n=6 each). Transferrin receptor (TFRC), tubulin (TUB), and Gapdh are loading controls and L = Ladder Lane. (J-Q) Super resolution images of 13-week-old control and TAL-cKO kidneys immunostained for UMOD and the endoplasmic reticulum (ER) tubule-associated protein reticulon 4 (RTN4). (J-M) In control kidney TAL cells, UMOD is enriched along the apical (luminal) membrane while RTN4 is present as intracellular puncta. (N-Q) In TAL-cKO kidneys, UMOD accumulates within TAL segments, the cells of which exhibit more intense RTN4 staining that appears to partially colocalize with intracellular UMOD, suggesting retention of UMOD within the ER. Scale bars: L, P = 20µm, M, Q (insets) = 5µm. Immunoblot results were normalized to controls in individual blots (n=3 per blot, n=3-6 total) and analyzed by unpaired t test with either Welch’s correction or Mann-Whitney test.

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Expressing, Western Blot, Control, Membrane, Staining, MANN-WHITNEY

NKCC2 protein expression and apical membrane localization are reduced in Myh9&10 TAL-cKO kidneys. (A-H) Fluorescence immunohistochemistry shows variable expression of NKCC2 in 9-week-old female and male Myh9&10 TAL-cKO (TAL-cKO) mice compared to littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ). (A, C) Expression of NKCC2 in control female TAL tubules is localized to the apical membrane. (B, D) Female TAL-cKO TAL segments show reduction in expression and apical membrane localization of NKCC2, some regions have more pronounced reduction in NKCC2 than others. (E, G) Male control kidneys also have robust expression of NKCC2 on the apical membrane of the TAL-segment. (F, H) TAL-cKO male kidneys have regions of NKCC2 expression that are drastically reduced than other regions of the kidney. Scale bars = 50µm. (n= 3 kidneys each). (I-N) Representative immunoblots for NKCC2 in male and female TAL-cKO and control littermate kidneys show progressive reduction of NKCC2. Quantification of immunoblots shows significant loss of NKCC2 in female TAL-cKO mice at 6, 9, and 13 weeks of age and in male TAL-cKO mice at 9 and 13 weeks of age compared to littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ), (n=6 each sex). Immunoblot results were normalized to controls in each blot and analyzed by unpaired t test with either Welch’s correction or Mann-Whitney test. Tubulin (TUB) is loading control and L= Ladder Lane. (O) Immunoblots of whole kidney lysates from female control (UMOD+/+ and UMODcre/+) and Myh9&10 TAL-cKO (TAL-cKO) mice at 6 weeks of age (upper panel) and 12 weeks of age (lower panel) showing NKCC2 protein levels appear unchanged at 6 weeks of age and only reduced in TAL-cKO kidneys compared to UMOD+/+ and UMODcre/+ kidneys at 12 weeks of age. (P) Quantification of immunoblots confirms no change in NKCC2 protein levels at 6 weeks of age and a significantly lower NKCC2 protein levels at 12 weeks of age in TAL-cKO kidneys compared to UMOD+/+ kidneys. No significant difference was observed in NKCC2 protein levels between UMOD+/+ and UMODcre/+ kidneys. Immunoblot results (O) were normalized to their respective controls (UMOD+/+, n=6 kidneys per age) and analyzed by one-way ANOVA with post hoc Dunnett T3 multiple comparisons test.

Journal: Function

doi: 10.1093/function/zqae048

Figure Lengend Snippet: NKCC2 protein expression and apical membrane localization are reduced in Myh9&10 TAL-cKO kidneys. (A-H) Fluorescence immunohistochemistry shows variable expression of NKCC2 in 9-week-old female and male Myh9&10 TAL-cKO (TAL-cKO) mice compared to littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ). (A, C) Expression of NKCC2 in control female TAL tubules is localized to the apical membrane. (B, D) Female TAL-cKO TAL segments show reduction in expression and apical membrane localization of NKCC2, some regions have more pronounced reduction in NKCC2 than others. (E, G) Male control kidneys also have robust expression of NKCC2 on the apical membrane of the TAL-segment. (F, H) TAL-cKO male kidneys have regions of NKCC2 expression that are drastically reduced than other regions of the kidney. Scale bars = 50µm. (n= 3 kidneys each). (I-N) Representative immunoblots for NKCC2 in male and female TAL-cKO and control littermate kidneys show progressive reduction of NKCC2. Quantification of immunoblots shows significant loss of NKCC2 in female TAL-cKO mice at 6, 9, and 13 weeks of age and in male TAL-cKO mice at 9 and 13 weeks of age compared to littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ), (n=6 each sex). Immunoblot results were normalized to controls in each blot and analyzed by unpaired t test with either Welch’s correction or Mann-Whitney test. Tubulin (TUB) is loading control and L= Ladder Lane. (O) Immunoblots of whole kidney lysates from female control (UMOD+/+ and UMODcre/+) and Myh9&10 TAL-cKO (TAL-cKO) mice at 6 weeks of age (upper panel) and 12 weeks of age (lower panel) showing NKCC2 protein levels appear unchanged at 6 weeks of age and only reduced in TAL-cKO kidneys compared to UMOD+/+ and UMODcre/+ kidneys at 12 weeks of age. (P) Quantification of immunoblots confirms no change in NKCC2 protein levels at 6 weeks of age and a significantly lower NKCC2 protein levels at 12 weeks of age in TAL-cKO kidneys compared to UMOD+/+ kidneys. No significant difference was observed in NKCC2 protein levels between UMOD+/+ and UMODcre/+ kidneys. Immunoblot results (O) were normalized to their respective controls (UMOD+/+, n=6 kidneys per age) and analyzed by one-way ANOVA with post hoc Dunnett T3 multiple comparisons test.

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Expressing, Membrane, Fluorescence, Immunohistochemistry, Control, Western Blot, MANN-WHITNEY

Mean serum sodium and potassium concentrations in female Myh9&10 TAL-cKO and littermate control mice. P values of less than 0.05 are shown in bold.

Journal: Function

doi: 10.1093/function/zqae048

Figure Lengend Snippet: Mean serum sodium and potassium concentrations in female Myh9&10 TAL-cKO and littermate control mice. P values of less than 0.05 are shown in bold.

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Control

Mean serum sodium and potassium concentrations in male Myh9&10 TAL-cKO and littermate control mice. P values of less than 0.05 are shown in bold.

Journal: Function

doi: 10.1093/function/zqae048

Figure Lengend Snippet: Mean serum sodium and potassium concentrations in male Myh9&10 TAL-cKO and littermate control mice. P values of less than 0.05 are shown in bold.

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Control

Sodium chloride cotransporter expression is differentially altered in female versus male Myh9&10 TAL-cKO kidneys. (A) Representative immunoblot from 9-week-old whole kidney lysates shows lower NCC expression in female Myh9&10 TAL-cKO (TAL-cKO) kidneys and higher expression in male TAL-cKO kidneys compared to littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ). The blot also confirms that female control mice have higher NCC levels compared to the male control mice. (B) Similar trends were observed for phosphorylated-NCC (pNCC) where TAL-cKO male kidneys had higher pNCC levels and female kidneys had lower pNCC levels compared to littermate controls. (C) Representative immunoblot from 13-week-old whole kidney lysates shows persistently higher NCC expression in male and lower NCC expression in female TAL-cKO kidneys compared to littermate controls. (D) Immunoblot from 13-week-old whole kidneys lysates showing pNCC expression in male and female control and TAL-cKO mice. Tubulin (TUB) and Gapdh are loading controls for the immunoblots and L = ladder Lane. (E) Quantification of NCC immunoblots shows significantly higher NCC expression in male TAL-cKO (triangles) kidneys and significantly lower NCC expression in female TAL-cKO (circles) kidneys at 9 weeks of age compared to littermate controls. No significant difference is seen in 13-week-old male or female TAL-cKO kidney NCC levels compared to littermate controls (n=3-6 each age and sex). (F) Quantification of pNCC immunoblots shows significantly higher pNCC expression in male TAL-cKO (triangles) kidneys and significantly lower pNCC expression in female TAL-cKO (circles) kidneys at 9 weeks of age compared to littermate controls. No significant difference is seen in 13-week-old male or female TAL-cKO kidney pNCC levels compared to controls (n=3-6). Immunoblot results were normalized to their respective controls in the blot and analyzed by unpaired t test with Welch’s correction or Mann-Whitney test (n=3 per blot, n=3-6 total). (G, H) Quantitative PCR of NCC (Slc12a3) gene expression shows no significant difference at 6, 9, or 12 weeks of age (n=3-6). Lack of significant difference in gene expression was determined by two-way ANOVA.

Journal: Function

doi: 10.1093/function/zqae048

Figure Lengend Snippet: Sodium chloride cotransporter expression is differentially altered in female versus male Myh9&10 TAL-cKO kidneys. (A) Representative immunoblot from 9-week-old whole kidney lysates shows lower NCC expression in female Myh9&10 TAL-cKO (TAL-cKO) kidneys and higher expression in male TAL-cKO kidneys compared to littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ). The blot also confirms that female control mice have higher NCC levels compared to the male control mice. (B) Similar trends were observed for phosphorylated-NCC (pNCC) where TAL-cKO male kidneys had higher pNCC levels and female kidneys had lower pNCC levels compared to littermate controls. (C) Representative immunoblot from 13-week-old whole kidney lysates shows persistently higher NCC expression in male and lower NCC expression in female TAL-cKO kidneys compared to littermate controls. (D) Immunoblot from 13-week-old whole kidneys lysates showing pNCC expression in male and female control and TAL-cKO mice. Tubulin (TUB) and Gapdh are loading controls for the immunoblots and L = ladder Lane. (E) Quantification of NCC immunoblots shows significantly higher NCC expression in male TAL-cKO (triangles) kidneys and significantly lower NCC expression in female TAL-cKO (circles) kidneys at 9 weeks of age compared to littermate controls. No significant difference is seen in 13-week-old male or female TAL-cKO kidney NCC levels compared to littermate controls (n=3-6 each age and sex). (F) Quantification of pNCC immunoblots shows significantly higher pNCC expression in male TAL-cKO (triangles) kidneys and significantly lower pNCC expression in female TAL-cKO (circles) kidneys at 9 weeks of age compared to littermate controls. No significant difference is seen in 13-week-old male or female TAL-cKO kidney pNCC levels compared to controls (n=3-6). Immunoblot results were normalized to their respective controls in the blot and analyzed by unpaired t test with Welch’s correction or Mann-Whitney test (n=3 per blot, n=3-6 total). (G, H) Quantitative PCR of NCC (Slc12a3) gene expression shows no significant difference at 6, 9, or 12 weeks of age (n=3-6). Lack of significant difference in gene expression was determined by two-way ANOVA.

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Expressing, Western Blot, Control, MANN-WHITNEY, Real-time Polymerase Chain Reaction

Epithelial sodium channel gamma subunit is expressed in Myh9&10 TAL-cKO medullary collecting duct s . (A) Immunostaining of 13-week-old control ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ) male kidney shows γENaC expression in calbindin- and AQP2-positive cells of the cortex and outer medulla. White boxes are enlarged regions in panels B-D. (B) γENaC is expressed in the cortical distal tubules and collecting ducts. (C) Little γENaC expression is observed in the outer medullary collecting ducts. (D) Inner medullary collecting ducts of control animals do not express γENaC. (E) Immunostaining of 13-week-old male Myh9&10 TAL-cKO (TAL-cKO) kidney sections shows higher γENaC expression in cortical epithelial cells and unexpected expression in the inner and outer medullary collecting ducts. White boxes are enlarged regions in panels F-H. (F) γENaC expression in TAL-cKO mice is higher in cortical distal tubules and collecting ducts. (G) γENaC expression is observed within the outer medulla of TAL-cKO mice. (H) γENaC expression is observed within the inner medulla of TAL-cKO mice (n=3-4 kidneys each). Scale bars in A,E=1000µm. Scale bars in B-D and F-H=25µm. (I) Western blot analysis of whole kidney lysates shows no difference in γENaC expression (~75kDa cleaved form) in 9-week-old male or female TAL-cKO mice compared to controls. Whole kidney lysates from 13-week-old male and female TAL-cKO mice show higher γENaC expression compared to controls. Ponceau stain was used for loading control assessment (see supplemental data). (J) Immunoblot quantification shows significantly higher γENaC protein in both male (triangles) and female (circles) 13-week-old TAL-cKO mice compared to controls (n=6 each sex). (K) γENaC ( Scnn1g ) gene expression is significantly higher in 9-week-old female TAL-cKO mice compared to controls and compared to 12-week-old TAL-cKO female mice. (L) Scnn1g gene expression is higher in 16-week-old male TAL-cKO mice compared to controls. Immunoblot results were normalized to controls and analyzed by age using unpaired t test with either Welch’s correction or Mann-Whitney test (n=3 per blot, n=3-6 total). Gene expression data was analyzed by two-way ANOVA with post-hoc Tukey’s test (n≥3 per condition).

Journal: Function

doi: 10.1093/function/zqae048

Figure Lengend Snippet: Epithelial sodium channel gamma subunit is expressed in Myh9&10 TAL-cKO medullary collecting duct s . (A) Immunostaining of 13-week-old control ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ) male kidney shows γENaC expression in calbindin- and AQP2-positive cells of the cortex and outer medulla. White boxes are enlarged regions in panels B-D. (B) γENaC is expressed in the cortical distal tubules and collecting ducts. (C) Little γENaC expression is observed in the outer medullary collecting ducts. (D) Inner medullary collecting ducts of control animals do not express γENaC. (E) Immunostaining of 13-week-old male Myh9&10 TAL-cKO (TAL-cKO) kidney sections shows higher γENaC expression in cortical epithelial cells and unexpected expression in the inner and outer medullary collecting ducts. White boxes are enlarged regions in panels F-H. (F) γENaC expression in TAL-cKO mice is higher in cortical distal tubules and collecting ducts. (G) γENaC expression is observed within the outer medulla of TAL-cKO mice. (H) γENaC expression is observed within the inner medulla of TAL-cKO mice (n=3-4 kidneys each). Scale bars in A,E=1000µm. Scale bars in B-D and F-H=25µm. (I) Western blot analysis of whole kidney lysates shows no difference in γENaC expression (~75kDa cleaved form) in 9-week-old male or female TAL-cKO mice compared to controls. Whole kidney lysates from 13-week-old male and female TAL-cKO mice show higher γENaC expression compared to controls. Ponceau stain was used for loading control assessment (see supplemental data). (J) Immunoblot quantification shows significantly higher γENaC protein in both male (triangles) and female (circles) 13-week-old TAL-cKO mice compared to controls (n=6 each sex). (K) γENaC ( Scnn1g ) gene expression is significantly higher in 9-week-old female TAL-cKO mice compared to controls and compared to 12-week-old TAL-cKO female mice. (L) Scnn1g gene expression is higher in 16-week-old male TAL-cKO mice compared to controls. Immunoblot results were normalized to controls and analyzed by age using unpaired t test with either Welch’s correction or Mann-Whitney test (n=3 per blot, n=3-6 total). Gene expression data was analyzed by two-way ANOVA with post-hoc Tukey’s test (n≥3 per condition).

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Immunostaining, Control, Expressing, Western Blot, Staining, MANN-WHITNEY

Acute amiloride treatment confirms higher ENaC activity in Myh9&10 TAL-cKO mice. (A-F) To assess ENaC function, 12-week-old female and male control and Myh9&10 TAL-cKO (TAL-cKO) mice were administered vehicle (-) or amiloride (+). (A) Sodium excretion is significantly higher in female TAL-cKO mice that received amiloride compared to those that received vehicle, while sodium excretion is not significantly different between control mice that received amiloride or vehicle. (B) Potassium excretion is significantly lower in female control mice treated with amiloride compared to those that received vehicle, but not significantly different between female TAL-cKO mice treated with amiloride and those that received vehicle. (C) Urine pH is higher in control and TAL-cKO females treated with amiloride compared to those receiving vehicle. (D) Sodium excretion is higher in male TAL-cKO mice treated with amiloride compared to those treated with vehicle only. No significant difference is seen in control animals treated with amiloride compared to vehicle. (E) Potassium excretion is significantly lower in control and TAL-cKO male mice receiving amiloride compared to those treated with vehicle. (F) Urine pH is significantly higher in amiloride-treated TAL-cKO male mice and is significantly lower in TAL-cKO vehicle mice compared to control vehicle mice. N ≥ 3 for each group in amiloride studies. Two-way ANOVA post-hoc Tukey’s test with multiple comparisons was used to analyze effects of genotype and amiloride treatment on urine parameters.

Journal: Function

doi: 10.1093/function/zqae048

Figure Lengend Snippet: Acute amiloride treatment confirms higher ENaC activity in Myh9&10 TAL-cKO mice. (A-F) To assess ENaC function, 12-week-old female and male control and Myh9&10 TAL-cKO (TAL-cKO) mice were administered vehicle (-) or amiloride (+). (A) Sodium excretion is significantly higher in female TAL-cKO mice that received amiloride compared to those that received vehicle, while sodium excretion is not significantly different between control mice that received amiloride or vehicle. (B) Potassium excretion is significantly lower in female control mice treated with amiloride compared to those that received vehicle, but not significantly different between female TAL-cKO mice treated with amiloride and those that received vehicle. (C) Urine pH is higher in control and TAL-cKO females treated with amiloride compared to those receiving vehicle. (D) Sodium excretion is higher in male TAL-cKO mice treated with amiloride compared to those treated with vehicle only. No significant difference is seen in control animals treated with amiloride compared to vehicle. (E) Potassium excretion is significantly lower in control and TAL-cKO male mice receiving amiloride compared to those treated with vehicle. (F) Urine pH is significantly higher in amiloride-treated TAL-cKO male mice and is significantly lower in TAL-cKO vehicle mice compared to control vehicle mice. N ≥ 3 for each group in amiloride studies. Two-way ANOVA post-hoc Tukey’s test with multiple comparisons was used to analyze effects of genotype and amiloride treatment on urine parameters.

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Activity Assay, Control

Journal: Function

doi: 10.1093/function/zqae048

Figure Lengend Snippet: Osmo-responsive genes show altered expression profile in Myh9&10 TAL-cKO kidneys and ENaC subunits are osmo-responsive in IMCD3 cells highlighting the cellular plasticity of collecting duct epithelium. (A) Elf5 gene expression is lower in female (circles) Myh9&10 TAL-cKO (TAL-cKO) kidneys at 12 weeks of age and in male (triangles) TAL-cKO kidneys at 9 and 12 weeks of age compared to control kidneys ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ). (B) Akr1b1 gene expression did not show statistically significant changes in female kidneys but trended lower in 9 weeks female TAL-cKO compared to control kidneys. In male TAL-cKO kidneys, Akr1b1 gene expression was significantly reduced at 9, and 12-weeks of age, which increased at 16 weeks compared to controls. (C) Ranbp3l expression is significantly lower in 6, 9, 12- and 16-week-old male TAL-cKO mouse kidneys while it varies in female TAL-cKO mice; no significant reduction was observed in female TAL-cKO mice compared to littermate controls ( Myh9 f/f ; Myh10 f/f ; Umod +/+ ), (n=3 each). Two-way ANOVA with post-hoc Tukey’s test was used to determine differences in gene expression between control and TAL-cKO mice. (D) IMCD3 cells cultured in 300mOsm media show detectable levels of Scnn1g (γENaC) gene expression. Scnn1g expression is significantly lower in cells cultured in 600mOsm media compared to those cultured in 300mOsm media. The expression of the αENaC and βENaC subunits are less influenced by changes in extracellular osmolarity. n=6-8 biological replicates. One-way ANOVA with post-hoc Dunnett’s T3 multiple comparisons was used for statistical analysis of gene expression.

Article Snippet: Sino Biological developed the polyclonal IgG custom antibody against mouse MYH9 protein using the amino acid sequence CSDEEVDGKADGADAKAAE.

Techniques: Expressing, Control, Cell Culture

Review

Structured Review

Images

Similar Products

Image Search Results